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The study investigated the use of multimedia in the teaching and learning of chemistry. A sample of 150 students and 23 chemistry teachers were drawn from a population of 3420 chemistry students and 59 chemistry teachers respectively in the FCT, Abuja senior secondary two using purposive sampling technique. Two research questions guided the study. Two instruments of Effect of Multimedia on Students’ Learning Questionnaire (EMCSLQ) and Chemistry Teachers Use of Multimedia Questionnaire (CTUMQ) were used to collect data. Frequency count and percentage were used to answer the research questions. The study result of the study revealed that 53.33% (80) of the respondents reported that the used of overhead projector in classroom teaching enhanced their understanding of chemistry concepts while 21.7% (5) and 26.1%(6) of the respondents agreed that chemistry teachers used projector in the teaching of chemistry. Based on the findings, it was concluded that there’s need for frequent use of multimedia in the teaching and learning of chemistry at the secondary school level. The study suggested that to make learning meaningful, teachers should always integrate multimedia in their classroom to enhance the understanding of chemistry.